IPL 2013: Kolkata Knight Riders edge home in low-scoring thriller
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Kallis was going steady at the other end, helping the hosts close in on the target. The South African veteran though, got out with KKR 20 short of pulling off a win.
After hitting Mithun for two fours in as many balls, Kallis, to KKR's fans' dismay, played one towards Moises Henriques who back pedalled to complete an easy catch.
Tiwary followed Kallis as the batsman played Vinay Kumar towards cover for Virat Kohli to pull off a difficult catch.
Needing five off the last over, Ryan ten Doeschate sealed the issue in KKR's favour with a boundary.
Earlier, on a slow wicket, RCB struggled to score freely.
Chris Gayle found the going tough, hitting just one four and a six for his 33, before being stumped off Narine's bowling.
AB de Villiers was the next best scorer with a 22-ball 28. Attempting a reverse sweep off Balaji immediately after hitting the paceman for a six in the 16th over, the South African found a faint edge and Bisla took a well-judged catch.
Apart form Gayle, Narine accounted for the wickets of Saurabh Tiwary (1), Rampaul (0) and Mithun (2).
Gambhir began with spinner Sachitra Senanayake and Balaji and the two bowlers provided a superb start by giving away just 16 runs in the first five overs. Senanayake dismissed Cheteshwar Pujara in the fifth over and Gayle was also subdued.
Narine and Kallis were introduced into the attack and they were successful in keeping Gayle and Virat Kohli quiet.
The runs came in ones and twos with KKR bowlers not allowing the batsmen to swing their arms freely.
Kohli and Gayle smashed ten Doeschate for 14 runs for RCB's best over. Kallis then dealt RCB a blow when he bowled Kohli, who dragged one back on to his stumps, as Bangalore managed just 54 for two in 10 overs.